multi-well tray sealer LK-2014

Description

The principle of enzyme substrate method

The enzyme substrate method uses coliform producing beta galactosidase to decompose the color source
substrate ONPG (Ortho-nitrophenyl-β-D-galactopyranoside) to make the culture medium change yellow, Escherichia
coli produces β -glucuronidase to decompose the fluorescent chromatic source substrate MUG（ 4-methyl- umbelliferyl-β-glucuronide）produce fluorescence under 366 nm ultraviolet, The principle is used to determine the
quality and quantity of total coliform bacteria, fecal coliform (heat coliform bacteria) and Escherichia coli in water.
When a few non target bacteria in the water sample try to react with ONPG or MUG to interfere with the
detection results, the matrix of the special formula in the colitech medium can inhibit the reaction process and
control the false positive. At the same time, these special formulations can effectively promote the reaction of the
biological enzymes and the fixed substrates of the target bacteria. The incidence of false negative is lower than that
of the traditional method. Colitech enzyme substrate test reagent is suitable for GB/T5750.12-2006 “drinking water
standard detection method” medium. The combined use of Colitech enzyme substrate reagent and 51 or 97
detection plates can be used to detect target bacteria in 100 ml water samples, the detection range of 51 detection
plates is 0 ~ 200MPN, and the detection range of 97 determination disk is 0 ~ 2419MPN, without dilution.

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