Enzyme substrate detection reagent

Description

Character  This product is white or light yellow granules

Clarification degree  Colorless or slightly yellow

pH  7.0～7.6

weight  2.7±0.5g

Storage  Long term storage, refrigerating, drying, sealing and avoiding light storage at 4 ~ 8 ℃。

Term of validity  1 years

Working principle

In the water samples containing total coliform bacteria, the target bacteria were cultured in the Minimal Medium 0NPG-MUG medium at 36±1 C. The specific enzyme beta galactosidase produced by the total coliform bacteria can decompose the color source substrate of the Minimal Medium 0NPG-MUG medium, which makes the culture medium yellow; meanwhile, the large intestine in the water sample is in large intestine. Escherichia coli produces a specific beta glucuronase to decompose the fluorescent substrate MUG in Minimal Medium 0NPG-MUG medium and produce characteristic fluorescence. The same principle, the heat coliform group (fecal coliform group) will decompose the color source substrate ONPG in the Minimal Medium 0NPG-MUG medium at 44.5±0.5 C, making the medium yellow.

Packaging specification  200/box

Usage method

Qualitative detection

Setp 1 Add reagent in 100ml water sample, dissolve it. and Culture 24h at 36±1℃

Step 2 Read result

Colorless = negative

Yellow = total coliform positive

Yellow = total coliform positive

Yellow + fluorescence = Escherichia coli positive

Quantitative detection

Note: the heat tolerant coliform group (fecal coliform) needs

to be cultured at 44.5± 0.5℃ 24h, and yellow is positive.

Quantitative detection

Step 1 Add reagent in 100ml water sample, dissolve it.

Step 2 Pour into the 51 or 97 detection plate.

Step 3 Use LK sealer to seal the detection plate, and cultivate 24h

at 36 ℃

Step 4 Results qualitative test was used to check the MPN table count.

Note: the heat tolerance coliform group (fecal coliform group) needs           to be cultured at 44.5±0.5℃ 24h, and the Yellow pore pattern           is positive, and the corresponding MPN table count is found

Please read the instructions carefully and use it