The LK-2010 program-controlled quantitative sealing machine and colitech enzyme substrate method detection reagent, LK detection plate, and LK quantitative bottle produced by Xi’an Like Environmental Protection Technology Co., Ltd. are used as a set of coliform enzyme substrate detection system. The program-controlled quantitative sealing machine is used for the qualitative and quantitative detection of total coliforms, fecal coliforms (heat-resistant coliforms) and Escherichia coli in water.。